Cell Structure and Quantitative Gram Stain of Bacillus megaterium

Cell Envelope Structure and Functions in Gram-negative Bacteria

Density, porosity, and structure of dried cell walls isolated from Bacillus megaterium and Saccharomyces cerevisiae.

Helium displacement and nitrogen adsorption techniques were used to determine the density and porosity, respectively, of freeze-dried cell walls isolated from Bacillus megaterium KM and Saccharomyces cerevisiae. The densities were 1.302 and 1.180 g/cm3, respectively, suggesting noncrystalline solids. The porosities were extremely small, indicating that the cell walls had collapsed and become es...

Ribonucleates and the Gram stain.

Salton (J. Gen. Microbiol. 30:223, 1963) summarized the evidence which demonstrates that the gram-positive cell is able to retain the crystal violet after iodine treatment because of the characteristic nature of its cell wall. This evidence is highly convincing, yet it does not explain the experiments of Henry and Stacy (Proc. Roy. Soc. London Ser. B 133:391, 1946) or of Bartholomew and Umbreit...

Crystal Structure of Germination Protease from Spores of Bacillus Megaterium

No. ponn1490 Beamline(s): X12C Cells of Bacillus and Clostridium species produce spores when one or more nutrients are limiting for growth of these organisms. The spores DNA is saturated with a group of small, acid-soluble proteins (SASP) of the α/βtype. In spore germination SASP are degraded to amino acids and this degradation is initiated by a sequence specific protease called the germination...

The Gram stain.